THE ANTIMICROBIAL ACTIVITIES OF Syzygium aromaticum OIL ON SOME SELECTED CLINICAL AND TYPED MICROORG

Abstracts

Syzygium aromaticum (cloves), are the aromatic dried flower buds of a tree in the family Myrtaceae. Cloves are native to Indonesia and used as a spice in food. The proximate, phytochemical analysis, antimicrobial activity, and minimum inhibition concentration of Syzygium aromaticum was determined. The oil of the dried flower bud was extracted using soxhlet apparatus. The antimicrobial analysis showed that Syzygium aromaticum dried flower bud oil has potent antimicrobial activity against all tested microorganisms. For methanol extract, the highest antibacterial activity was found against Escherichia coli NCIB 86(27.3±2.08 mm), Staphylococus aureus NCIB 950(27±1.73 mm), Salmonella typhi NCIB 6539(27.3±1.52 mm), Bacillus cereus NCIB 6344(27.7±0.58 mm), followed by Pseudomonas aeruginosa ATCC 27853 (25.7±0.58 mm), Candida albicans (23±1.53 mm),and least against Klebsiella pneumonia (14.7±0.58 mm). For acetone extract, the highest antibacterial activity was found against, Escherichia coli NCIB 86 (27.3±2.08 mm), Staphylococus aureus NCIB 950 (27±1.73 mm), Salmonella typhi NCIB 6539 (27.3±1.52 mm), followed by Klebsiella pneumoniae ATCC 29522 (25.3±0.58 mm), Pseudomonas aeruginosa (25.3±1.53 mm), Candida albicans (22±3.46 mm), and least against, Pseudomonas aeruginosa (19±1.73 mm), and Klebsiella pneumoniae (17.7±0.58 mm) for inhibition zone diameter at lower and higher oil concentrations, respectively. The minimum inhibitory concentration was at 40 µg/ml, 20 µg/ml and 10 µg/ml for methanolic extract, while minimum inhibitory concentration for acetone extract was at concentration of 20 µg/ml to 10 µg/ml only Pseudomonas aeruginosa was at 40 µg/ml. This study has shown the importance of Syzygium aromaticum flower bud and indicated that, the plant can be used as an antibacterial agent and it is edible with high nutritional contents.

Keywords : Syzygium aromaticum; cloves; proximate analysis; phytochemical analysis; antimicrobial activity; minimum inhibition concentration (MIC).

Please see more information