ANTIBACTERIAL EFFICACY OF SOME STANDARD ANTIBIOTICS DISCS COATED WITH BIOLOGICALLY SYNTHESIZED SILVE

In the field of medicine, biologically generated silver nanoparticles are widely used. Cassia occidentalis leaf extracts were used to reduce aqueous silver ions in the brief phase of extracellular production of silver nanoparticles. The goal of this study is to see how effective typical antibiotic discs coated with biologically produced silver nanoparticles from Cassia occidentalis leaves are against bacteria. Tannins, flavonoids, saponins, glycosides, and triterpenoids were discovered in preliminary phytochemical investigation of aqueous extracts. The colour change of plant extracts from yellow to dark brown confirmed the creation of silver nanoparticles. Antibacterial activity was examined using the disc diffusion method after standard antibiotic discs were coated with phytosynthesized silver nanoparticles at varied doses. S. typhi, P. aeruginosa, K. pneumoniae, and E. coli are among the test cultures. The zone of inhibition was used to assess the antibacterial activity of conventional antibiotic discs coated with phytosynthesized silver nanoparticles. The results indicated that S. typhi, K. pneumoniae, P. aeruginosa, and E. coli are sensitive; similarly, the MIC result indicates that S. typhi and E. coli are sensitive at 250 g/ml concentration, whereas K. pneumoniae and P. aeruginosa are sensitive at 500 g/ml; the MBC result indicated that E. coli, S. typhi, and K. pneumoniae are sensitive When compared to ordinary antibiotics alone, antibiotic discs coated with phytosynthesized AgNPs had better antibacterial activity against the bacterial isolates.

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