CHARACTERIZATION AND COMPARATIVE RESPONSE OF RHIZOBIA ISOLATES FROM ROOT NOODLES OF SELECTED WEED ..

For biological nitrogen fixation, screening and selecting the most effective rhizobium strains is critical. Rhizobia strains isolated from root nodules of five weed species (Mimosa pudica, Crotolaria retusa, Desmodium triflorum, Canavalina ensiformis, Stylosanthes spp.) were subjected to varying temperature, salinity, and pH in the laboratory to determine the effects of environmental factors on rhizobia strains associated with some selected leguminous weed species. When comparing temperatures of 30 and 370 degrees Celsius to temperatures of 250 degrees Celsius, the results showed that temperatures of 30 and 370 degrees Celsius resulted in superior growth. Microbial growth was also shown to be best at a salt concentration of 1.5 percent. The pH range of 5 to 8 was also found to be optimal for growth. A series of morphological and biochemical tests found that all of the strains were gram-negative, rod-shaped, and mucous generating, according to the study. In all of the circumstances tested, rhizobia strains from leguminous weeds grew faster than the nodulating soybean strain. To determine the compatibility of these wild rhizobial strains with domesticated leguminous plant species, a cross inoculation study was advised.

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