COLORIMETYRIC ASSAY OF TOBRAMYCIN SULPHATE IN BULK AND IN DOSAGE FORMS | Journal of Applied ......

For the assay of tobramycin sulphate, three rapid, accurate, and sensitive visual spectrophotometric techniques have been developed. Tobramycin is reacted with 3-methyl-2-benzothiazolinone hydrazone hydrochloride in the presence of ceric ammonium sulphate in Method A (max; 470 nm). The reaction with p-dimethylaminobenzaldehyde in the presence of sulphuric acid and ferric chloride is used in Method B (max; 505 nm). The development of a coloured ion-pair complex between tobramycin and rose bengal in Method C (max; 579 nm) is based on the formation of a coloured ion-pair complex between tobramycin and rose bengal. The reaction mechanisms have been presented and all variables have been optimised. In the concentration ranges of 1.0-12, 1.5-30, and 3.5-71 g/mL, regression analysis of the Beer's plots revealed good correlation for methods A, B, and C, respectively. Excipient interference was not seen, and the methodologies' validity was validated by assessing pharmaceutical dosage forms containing tobramycin. Within 1.42 percent, the relative standard deviations were all within 1.42 percent. Ringbom's optimum concentration ranges for more accurate results are 1.5-11, 3.0-27, and 7.0-68 g mL, respectively. Also estimated are apparent molar absorptivity, Sandell sensitivity, detection, and quantification limits.

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