COMPARATIVE PROTEOMIC STUDY OF LETTUCE LEAF USING MULTI-DIMENSIONAL PROTEIN IDENTIFICATION TECHNO...

Plant factories are a sensible choice for long-term food production. However, when artificial lighting is used, it consumes a lot of energy and costs a lot of money. Because of the inhibition of the thylakoid multiprotein complex and photosynthetic metabolism under lighting conditions, light quality has a significant impact on plant growth and quality. This phenomenon's molecular mechanism is currently unknown. The goal of this study was to look at the first comparative quantitative proteomic analysis of lettuce in a closed biological production system under light quality. We looked at how the lettuce proteome reacted to three different light treatments (R:B ratio of 1.2 and 2.2 provided by LEDs, and R:B ratio of 1.8 provided by the fluorescent lamp). Mass spectrometry and bioinformatics, as well as the Mascot database, were utilised to test the rapid identification and sensitive detection of protein expression in response to light quality. Our findings showed that light quality had a substantial impact on changing the proteome level and expression pattern in lettuce leaves, as well as a considerable impact on functional, molecular, and biological levels in comparison to other light treatments. The study was successful in identifying seventy-one proteins involved in energy metabolism and morphogenesis in lettuce plants treated with fluorescent lamps, fifty and forty-one proteins for LEDs, respectively. Due to the effect of spectrum light quality differences, the molecular mechanisms of key enzymes in thylakoid complex proteins and photosynthetic metabolism were substantially altered among light treatments. Importantly, lettuce plants express less proteins that are diagnostic for LED light. In this regard, our findings will serve as a springboard for further research into the benefits of this next-generation lighting technology, as well as the link between light quality and the multi-complex protein (CB23 HORVU) of the LHC family in extremely energy-efficient horticulture.

Please see the link :- https://www.ikprress.org/index.php/AJOPSS/article/view/3371