DETECTING DNA POLYMORPHISM ON MULBERRY (Morus Sp.) USING RAPD AND ISSR MARKERS | PLANT CELL BIOTECH

Mulberry molecular analysis has not been widely reported in Indonesia, thus more research into molecular genetics is needed. The DNA polymorphism markers Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were found to be reliable approaches for distinguishing variations and revealing genetic diversity of three provenances in South Sulawesi. This work attempts to determine the potential for polymorphisms in mulberry plants using RAPD and ISSR markers, and it is deemed highly significant to be used as basic knowledge in future mulberry plant breeding and conservation efforts. On mulberry, the outcomes of primary selection using RAPD markers. Primers OPK-20, OPA-15, OPA-05, OPAD-11, OPG-19, OPAE-11, OPP-08, and OPD-20 successfully amplified 8 out of 10 primers with unambiguous polymorphic bands. The key ISSR 10 markers, UBC 810, UBC 813, UBC 814, UBC 820, UBC 822, UBC 823, UBC 824, UBC 827, UBC 830, and UBC 868, were effectively amplified with polymorphic and bright bands and may now be utilised for mulberry diversity study.

Please see the link :- https://www.ikprress.org/index.php/PCBMB/article/view/6309