DETERMINATION OF METABOLIZABLE ENERGY OF SORGHUM SILAGE WITH DIFFERENT LEVELS OF FIBROLYTIC ENZY....

Using the gas production approach, the fermentation properties and metabolizable energy (ME) of sorghum silage with various amounts of fibrolytic enzymes were determined. To compare the additional time of enzyme (before or after ensiling) with three different levels of enzymes (3g, 6g, and 9g) in sorghum silage, three rumen fistulated Holstein steers were employed in a complete random design with a 2 x 3 factorial arrangement of treatments. Sorghum silage, B: sorghum + 3 g fibrolytic enzymes before silage, C: sorghum + 6 g fibrolytic enzymes before silage, D: sorghum + 9 g fibrolytic enzymes before silage, E: sorghum + 3 g fibrolytic enzymes after silage, F: sorghum + 6 g fibrolytic enzymes after silage, and G: sorghum + 9 g Gas production characteristics (b and c) were reported using the equation y = b + c after 2, 4, 6, 8, 12, 24, 48, 72, and 96 hours of incubation (1-e-ct). The results revealed that enzymes reduced the quantity of Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) in silage (P0.05) while increasing the content of Crud Protein (CP), Ether Extract (EE), and Water Soluble Carbohydrate (WSC) (P>0.05). Treatment G had a higher amount of gas production at each incubation time than the others. Additional enzyme after ensiling resulted in a considerable reduction in ADF and NDF concentration in treatment G. The enzyme increased (b) fraction, but this rise was not significant in any of the treatments, which is likely owing to the tannins in sorghum, which limit methane production. There was a significant difference in fraction (c) between the treatments. After 24 hours, G had the highest gas production and ME (P0.05) compared to all other treatments. It was determined that silage and enzymes can be used in the composition of ruminant diets.

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