DNA METHYLATION STATUS IN LEAF, ROOT AND RHIZOME OF Valeriana jatamansi | Journal of Global Agricul

In plants, DNA methylation, an important epigenetic mark, is recognised to have a crucial function in regulating gene expression. It's also known to be a crucial regulator for a variety of processes in many tissues. Using a methylation sensitive amplification polymorphism (MSAP) technique, variations in DNA methylation pattern of CCGG sites were examined in leaf, root, and rhizome tissues of Valeriana jatamansi, a medicinally significant plant. DNA polymorphism in terms of methylation was assessed using a combination of eight selective MSAP primers. There was a significant shift in MSAP profiles of genomic DNA of this medicinally essential plant. The root (16.82%) has the highest methylation, followed by the rhizome (15.48%) and the leaf (12.37%) tissues. The sequences of five methylation-altering MSAP segments that were differentially amplified were successfully retrieved. In these three tissues, there were more than 15 MSAP band variations. Only two differentially amplified MSAP fragments were sequenced and identified using BLAST analysis in leaf tissue (LM-1 and LM-2) and root tissue (RM-1 and RM-2). In rhizome tissue, one differently amplified MSAP fragment (RHM-1) was characterised. The findings show that different MSAP fragments in different tissues may be responsible for Valeriana jatamansi's physiological response control.

Please see the link :- https://www.ikprress.org/index.php/JOGAE/article/view/617