ESTIMATION OF WEDELOLACTONE IN Eclipta prostrata (L.) L BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AF

Wedelolactone levels in methanolic extracts of E. prostrata were determined using Bacillus megaterium (B), Trichoderma harzianum (T), Funneliformis mosseae (F), and consortia of F.mosseaeand T. harzianum (FT), 5. B. megaterium and T. harzianum (BT), 6. F. mosseae and B. megaterium (FB The process described above produces repeatable, high-resolution, reliable, and cost-effective results. Under both drought and irrigated soil conditions, the wedelolactone level in E. prostrata was found to be higher after treatment with microbial consortia of Funelliformis mosseae and Bacillus megaterium (FB). In comparison to irrigated soil, drought increased the concentration of wedelolactone in plants. After treating FB with drought stress, the maximum amount of wedelolactone was discovered.

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