INSECTICIDAL AND TOXICOLOGICAL STUDY OF Dennettia tripetala (G. Baker) FRUIT OIL EXTRACT USED AS ...

The insecticidal properties of Dennettia tripetala fruit ethanolic oil extract were examined. The bioassay was carried out using 0.2, 0.4, 0.6, 0.8, and 1.0 ml/20 g of sensitive cowpea. The 1.0 ml dosage was shown to be the most effective, as it caused 100 percent insect mortality within 24 hours of application and was substantially (p0.05) different from the other treatments, with the exception of the positive control, which caused up to 94 percent beetle mortality. When compared to the untreated control, all dosages had a significant fatal effect on the insect. The mortality of insects was dose dependant. Fruit oil extract required 2.46 and 6.66ul to produce 50 and 95 percent insect death within 24 hours, according to probit analysis. Treated seeds with the maximum dose of 1.0 mL D. tripetala extract inhibited oviposition, adult emergence, and the insect's ability to cause damage and weight loss, resulting in less infestation and consequent damage to treated cowpea seeds. Thus, Dennettia tripetala extract could be a possible toxin and play an important role in protecting stored cowpea from bruchids during storage. As the dosages were raised, the levels of bilirubin, GGT, and Uric acid rose. When compared to the other groups, the positive control had considerably greater levels of Bilirubin (69.11g/dl), GGT (38.02g/dl), and Uric acid (50.25g/dl), while Total protein was at its lowest (0.06g/dl). The low quantities of AST (Aspartate Aminotransferase), ALP (Alkaline Phosphate), ALT (Alanine Aminotransferase), and Creatine found in 1.0ml of 2% D. tripetala oil extract have no hepatotoxic effect on animals.

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