ISOLATION OF ACTINOMYCETES FROM RHIZOSPHERE SOIL: A COMPLETE APPROACH | PLANT CELL BIOTECHNOLOGY ...

The aim of this study is to isolate actinomycetes from a variety of rhizosphere soil samples using various pretreatments and selective media. Methods: A actinomycetes strain was isolated from rhizosphere soil samples of various plants using soil pretreatment, physical and chemical treatment, and growth with and without antibiotic medium.Results: Physical and chemical pretreatments, such as dry heat, centrifugation, phenol treatment, enrichment in a nutrient medium, calcium carbonate treatment, and selective nutrient medium with and without antibiotics, were used to separate 69 actinomycetes strains from rhizosphere soil samples. The isolation of actinomycetes is affected by physical treatment and chemical concentration. Dry heat at 40°C is found to be an efficient pretreatment, centrifugation removes unwanted soil debris, phenol and calcium carbonate are more effective chemical treatments, and antibiotics medium is useful for actinomycetes isolation by inhibiting bacterial and fungal development. Actinomycetes have been discovered to be abundant in rhizosphere soil and to be capable of producing plant growth hormones, bio-nutrients, and bioactive compounds that are beneficial to soil fertility and plant growth.

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