MOLECULAR EVALUATION AND PHENOL APPLICATION EFFECTS ON BARLEY INFESTATION BY Rhyzopertha dominica...

Because of pre- and post-harvest losses of agricultural goods around the world, food safety has been a problem for many years. Twelve barley cultivars were used in this study to evaluate grain yield, quality parameters, and insect pest (Rhyzopertha dominica (F.) infestation using Sequence-Related Amplified Polymorphism (SRAP) marker analysis to study genetic diversity among the 12 cultivars and Phenol as a chemical application such as modern methods of stored grain protection. The presence of significant genetic information variations among all cultivars and their responses to the insect pest (R. dominica) was confirmed using SRAP markers (F.). Primer (me6+em6) identified a lot of polymorphism (83%) and a lot of polymorphic information content (PIC) (0.84%). Based on their genetic information, the dendrogram of SRAP markers had clustered all the cultivars into two primary groups, which could be used in cultivar identification breeding for insect pest management. Hulless barley was more vulnerable to R. dominica because it had a high protein and low phenol content. After five days of exposure, the application of phenol concentration (0.4 g ai/kg) in all the test hulless grain caused total decrease of adult progeny of insect Giza 135 and least damage to grains, with the lowest Lethal Concentration 50 percent (LC50 percent) being 0.33 g ai/kg grain. Furthermore, no changes in technological grain qualities were seen, indicating that employing phenol will be a beneficial aspect of integrated pest management (IPM) programmes.

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