REGENERATION FROM MATURE SCUTELLUM EXPLANTS OF RICE VARIETY IR64 (Oryza sativa L.) THROUGH DIRECT...

For direct and indirect regeneration from mature scutellum explants of rice (Oryza sativa) variety IR64, an efficient approach was designed. For germination, mature dehulled surface sterilised seeds were deposited on Murashige and Skoog's basal medium. For callus induction, scutellum was dissected from aseptically grown, five-day-old seedlings. Scutellum explants produced excellent yellow calli. The proportion of callus induction ranged from 60 to 98 percent. In MS basal medium containing 3.0 mg L-1 2, 4-D, the maximum frequency of callus induction was found. The mean percentage of regeneration ranged from 17.5 to 67.5 percent in the presence of BAP, with a mean shootlet count of 10 per callus. In the presence of 2.5 mg L-1 BAP, both the frequency of green shoots and the quantity of shoots generated per callus were high. In direct regeneration, MS basal medium supplemented with 1.5 mg L-1 TDZ was found to be extremely helpful for seed germination and multiple shoot development (57.2 shoots). In MS media containing 0.75 mg L-1 NAA, thick, white, and fibrous roots were formed. Direct regeneration shoots produced a higher number of roots (18.7) and longer roots (11.7 cm). The in vitro regenerants developed well and shape and growth characteristics showed no discernible difference. Plants that had been regenerated in vitro had been successfully transplanted to the soil. Explants of Scutellum from IR64 mature seeds could be employed for molecular breeding.

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