REGENERATION FROM MATURE SCUTELLUM EXPLANTS OF RICE VARIETY IR64 (Oryza sativa L.) THROUGH DIRECT AN

Direct and indirect regeneration from mature scutellum explants of rice (Oryza sativa) variety IR64 was established using an effective procedure. For germination, mature dehulled surface sterilised seeds were deposited on Murashige and Skoog's basal medium. For callus induction, scutellum was dissected from aseptically grown, five-day-old seedlings. Scutellum explants produced excellent yellow calli. The proportion of callus induction ranged from 60 to 98 percent. The MS basal medium with 3.0 mg L-1 2, 4-D had the highest incidence of callus induction. The mean percentage of regeneration varied from 17.5 to 67.5 percent in the presence of BAP, with a mean shootlet count of 10 per callus. In the presence of 2.5 mg L-1, both the frequency of green shoots and the quantity of shoots generated per callus were high. BAP. In direct regeneration, MS basal medium supplemented with 1.5 mg L-1 TDZ was shown to be extremely helpful for seed germination and multiple shoot growth (57.2 shoots). In MS media containing 0.75 mg L-1 NAA, thick, white, and fibrous roots were formed. Direct regeneration shoots generated a higher number of roots (18.7) and longer roots (11.7 cm). The in vitro regenerants developed well and shape and growth parameters showed no discernible difference. In vitro-grown plants were successfully transplanted into the wild. Explants of Scutellum from IR64 mature seeds might be employed for molecular breeding.

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