STACHYIBOTRUS TOXIC MICROSCOPIC FUNGUS LOW MOLECULAR METOBOLITES | PLANT CELL BIOTECHNOLOGY AND....

Ethyl acetate was used to extract the Stachybotrys chartarum strain's filtered culture fluids. Column chromatography (silica gel) with gradient elution was used to separate the isolated red residue into two fractions. A minor amount of a combination of recognised stachybotrolide compounds was obtained by TLC on the opposite side of fraction 1. The chromatographic and spectroscopic characteristics of these compounds were comparable to those of standard samples. Fraction 2 was a crystalline substance with the same 1H and 13C characteristics as stachibotrin, a driman's sesquiterpenoid derivative. The hypothesised structure for this chemical was based on spectroscopic data, and no evidence of the stereogenic carbon atom arrangement was obtained. An X-ray diffraction investigation of a single crystal was used to describe this metabolite. The fungus was primarily formed by a fully described stachybotrys derived sesquiterpenoid of dromana.

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