STANDARDIZATION OF in-vitro REGENERATION FOR Gynura procumbens (LOUR.) MERR | Journal of Global ...

From July 2016 to April 2017, the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University (PSTU) conducted an in vitro regeneration study of Gynura procumbens L. in order to develop a standardised low-cost protocol for the organogenesis of this species. The intermodal segment was employed as the explant in this investigation, and the treatments were Germplasm pond water + Agar 7g+ Sucrose 30g, Tarangotanu pond water + Agar 7g+ Sucrose 30g, 14 MS, 1/2 MS, and MS medium. The control was modified traditional MS medium (cm), whereas table sugar was employed as an alternative source of carbon. With the full strength of MS medium, the maximum shoot initiation percentage (73.64), the largest number of shoots per plantlet (12.45), and the maximum shoot length (3.19 cm) were reported at 45 DAC. The 14 strength of MS medium yielded the lowest number of days (13.69) for shoot initiation and the largest number of leaves per shoot (12.52). The medium containing Germplasm pond-water, on the other hand, yielded the shortest days (12.30) for root initiation and the maximum root initiation percentage (83.03), the highest number of roots per plantlet (10.47), and the maximum root length (6.21 cm). The rooted plantlets were placed in polybags with a mixture of garden soil, sand, and cow dung for acclimatisation (1:1:1). The plantlet treated with Germplasm pond water media had the maximum survivorship (80.53 percent), as well as the best economic return (BCR). This research has shown that by using alternate nutrition sources with potential applications for other herbaceous plants, it is possible to lower the cost of tissue culture.

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