STATISTICAL OPTIMIZATION FOR COMPETITIVE BIOSORPTION OF REACTIVE ORANGE 13 AND REACTIVE BLUE 222 ...

This work used sequential statistically designed experiments to investigate the competitive biosorption of Reactive Orange 13 and Reactive Blue 222 from aqueous solution using Rhizopus arrhizus dead biomass as a cost-effective fungus biosorbent. In the first step, the Plackett-Burman design was utilised to determine the most important elements impacting dye biosorption. In the second step, Response Surface Methodology was used to find circumstances that would result in maximal biosorption. The second-order polynomial models were extremely significant in both dyes, with P 0.0001 and strong coefficients of determination, according to ANNOVA. pH 2.5, dye concentration 100 mg L-1, biosorbent dosage 1.7 g L-1, temperature 35°C, agitation speed 80 r min-1, and contact time 120 min were used to achieve maximal sorption (93.76 percent for Reactive Orange 13 and 97.83 percent for Reactive Blue 222). The time-dependent elimination of colour by the biosorbent was used to evaluate kinetics and isotherms. FTIR, XRD, and BET measurements were used to reveal the biosorption mechanism. The possibility of using Rhizopus arrhizus as a biosorbent to remove colours from a binary combination was proven in this study.

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