SYNTHESIS AND CHARACTERIZATION OF PEGylated REDUCED GRAPHENE OXIDE: DETERMINATION OF TOXICITY .....

Graphene is a new type of carbon-based nanomaterial with a number of distinct physicochemical properties. These characteristics are being used in biomedicine, particularly in stem cell regenerative treatment. It is employed as a coating for tissue engineering scaffolds because of its high mechanical strength, capacity to induce stem cell differentiation and proliferation, and antimicrobial qualities. However, there is a paucity of research on graphene's biocompatibility. The goal of this research is to make PEGylated reduced graphene oxide (PrGO) and see how well it works in bone marrow mesenchymal stem cells (MSCs). TEM, SAED, AFM, XPS, Raman spectroscopy, and FTIR were used to evaluate PrGO, which was made by reducing graphene oxide. To determine the stemness of MSCs, they were characterised. MTT assay was performed on the described MSCs after they were subjected to varied concentrations of PrGO. PEG was successfully coated on to rGO, and MSCs were found to retain their stemness in vitro. Furthermore, PrGO was discovered to be biocompatible and to promote MSC proliferation. Finally, when in-house synthesised PrGO was exposed to MSCs, it was determined to be non-toxic. This research could aid in the development of PrGO for biomedical applications.

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