THE USE OF PHOSPHOMOLIBDATE METHOD FOR DETERMINATION OF ANTIOXIDANT ACTIVITY IN MILK EXTRACT .....

Antioxidants are primarily non-nutrient substances found in both human and animal diet, but they have the antioxidant capacity in vitro to offer an artificial power index by suppressing nutrient oxidation and thereby preventing the death of cells and tissue potential. Antioxidants, both lipophilic and hydrophilic, are found in milk and play an important role in its preservation. The method used to determine the antioxidant activity of milk matches to the method used to determine the antioxidant activity of plants, and there is a significant linear positive association. The phosphor molybdenum technique, which is based on the reduction of Mo (VI) in Mo (B) in milk samples, is used for this purpose. Trichloroacetic acid extracts (6% trichloroacetic acid) (TCA). At an acidic pH value, green compounds develop and are measured spectrophotometrically in the UV spectrum at wavelength = 695 nm. The milk sample data were used to create a gallic acid calibration curve. Four varieties of milk were examined for antioxidant activity, with ten extracts per sample and Excel statistics. In comparison to ordinary commercial milk, the findings were lower. The conclusion is that packaged milk with 3.2 percent fat has the maximum antioxidant activity, which is attributable to the effects of lipids, the presence of vitamin E, and their synergistic impact with vitamin C. On the other hand, we conclude that the highest total antioxidant activity in milk, the first sample, is due to the use of several types of animal feed, including alfalfa, two concentrates, and straw, demonstrating that antioxidant activity is dependent on the impact of nutrients, i.e. their type and quantity.

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