TITRIMETRIC AND SPECTROPHOTOMETRIC ASSAY OF REPAGLINIDE IN PHARMACEUTICALS | Journal of Applied ...

The antidiabetic medication repaglinide (RPG) is used to treat type 2 diabetes. For the determination of RPG in bulk drugs and tablets, one titrimetric and two spectrophotometric approaches are proposed. Titrimetry is used to titrate a drug solution in anhydrous acetic acid with acetous perchloric acid to a crystal violet end-point (titrimetric method). At 400 and 425 nm, spectrophotometry measures highly coloured ion-pair complexes generated on interacting RPG with bromocresol purple (BCP) (SP-BCP technique) and bromocresol green (BCG) (SP-BCG method) in dichloromethane medium. The effects of various experimental conditions on the assays were thoroughly investigated and incorporated into the protocols. RPG was discovered to have a 1:1 reaction with perchloric acid, which was used as the basis for titrimetry estimates in the 1-10 mg range. Beer's law was observed in the range of 1-16 g/mL for both techniques, with molar absorptivity values of 1.16 103 and 1.57 103 L/mol/cm for BCP and BCG, respectively, under the given conditions. For the BCP technique, the limits of detection (LOD) and quantification (LOQ) were calculated to be 1.94 and 5.85 g/mL, respectively, and for the BCG method, 0.95 and 2.85 g/mL. The drug-reagent reaction was discovered to have a 1:1 stoichiometry using Job's method of continuous variations. The intra-day and inter-day percent RSD values were both less than 2.02, and the corresponding percent RE values were less than 2.33. The created methods were used to determine RPG in tablets, and the results were satisfactory, with a 2 percent inaccuracy. Recovery studies using the standard-addition process were used to test accuracy, and the methods were shown to be free of interference from co-formulated drugs.

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