TYPE III-A CRISPR/CAS SYSTEMS AND COMPARISON OF CAS1, CAS2, AND CAS10 PROTEINS OF LACTOBACILLI | ..

In lactic acid bacteria, type I and type II CRISPR systems are the most common, but type III is found in a few genera. Lactobacilli with a type IIIA CRISPR system were identified and their Cas10 signature nucleases, Cas1, Cas2 proteins, and repeat sequences were compared in this study. Both lactobacilli in 39 strains from 9 species had the Type IIIA CRISPR system. Of all species, Ligilactobacillus salivarius strains had the most type IIIA systems. The most common repeat sequence was “agtaagaacatatctccgaatataggagacgaaac,” and the secondary structure was predicted using the RNAfold analysis method.The phylogenetic tree and amino acid sequence alignment of Cas1, Cas2, and Cas10 showed that the strains appeared to break into speciesspecific divisions. Between cas6 and CRISPR series, L. ruminis ATCC 27782 and ATCC 27780 had IS66 family insertion sequence element accessory protein TnpB, as well as DEDDh genes. Type III-A CRISPR systems' endogenous Cas10 enzyme has recently been used in genetic engineering experiments, and reprogrammed type IIIA systems have been tested for endogenous genome editing.As a result, this study retraced lactobacilli type IIIA CRISPR systems to assess clustering of repeat sequences, Cas1, Cas2, and Cas10 proteins among strains, as well as to identify lactobacilli as a candidate for future reprogrammed endogenous genome editing.

Please see the link - https://ikprress.org/index.php/AJMAB/article/view/6120