UPGRADATION ON THE ROLE OF SPERMINE THROUGH in vitro CULTURE STUDY ON REGENERATION, GROWTH AND DEVEL

The effect of different polyamine concentrations on Sargassum jhonstonii in vitro cultivation was investigated. In solid media supplemented with 10-6 M spermine, the disorderly mass of cells was noticed, and they eventually produced two blade-like outgrowths. After 72 hours of inoculation, the initial regeneration was detected in PES medium supplemented with 10-6M spermine. Early in the regeneration process, cells at the cut ends become mitotically active and undergo cell differentiation, resulting in filamentous outgrowths. Several primary and secondary lateral branches of leaves and stipes grew from these filamentous outgrowths. Two elongated stipes about 3.5 cm in length formed from the midrib area. Though different concentrations of spermidine and putrescine plus f2 and ESW resulted in growth and regenerated stipes, the rate of growth was inadequate. However, a whole plant with multiple leaves, branches, and receptacles measuring 6.5 cm in length grew well in PES medium supplemented with 10-6 M spermine after 10 weeks of inoculation.

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